diff --git a/erpnext/stock/doctype/item/test_item.py b/erpnext/stock/doctype/item/test_item.py
index a0ed1f2..2b3d3b7 100644
--- a/erpnext/stock/doctype/item/test_item.py
+++ b/erpnext/stock/doctype/item/test_item.py
@@ -32,7 +32,7 @@
 test_dependencies = ["Warehouse", "Item Group", "Item Tax Template", "Brand", "Item Attribute"]
 
 
-def make_item(item_code=None, properties=None, uoms=None):
+def make_item(item_code=None, properties=None, uoms=None, barcode=None):
 	if not item_code:
 		item_code = frappe.generate_hash(length=16)
 
@@ -61,6 +61,14 @@
 		for uom in uoms:
 			item.append("uoms", uom)
 
+	if barcode:
+		item.append(
+			"barcodes",
+			{
+				"barcode": barcode,
+			},
+		)
+
 	item.insert()
 
 	return item
diff --git a/erpnext/stock/doctype/stock_entry/stock_entry.py b/erpnext/stock/doctype/stock_entry/stock_entry.py
index 9bb607c..238de34 100644
--- a/erpnext/stock/doctype/stock_entry/stock_entry.py
+++ b/erpnext/stock/doctype/stock_entry/stock_entry.py
@@ -30,6 +30,7 @@
 	OpeningEntryAccountError,
 )
 from erpnext.stock.get_item_details import (
+	get_barcode_data,
 	get_bin_details,
 	get_conversion_factor,
 	get_default_cost_center,
@@ -428,7 +429,14 @@
 			for field in reset_fields:
 				item.set(field, item_details.get(field))
 
-			update_fields = ("uom", "description", "expense_account", "cost_center", "conversion_factor")
+			update_fields = (
+				"uom",
+				"description",
+				"expense_account",
+				"cost_center",
+				"conversion_factor",
+				"barcode",
+			)
 
 			for field in update_fields:
 				if not item.get(field):
@@ -1634,6 +1642,10 @@
 			if subcontract_items and len(subcontract_items) == 1:
 				ret["subcontracted_item"] = subcontract_items[0].main_item_code
 
+		barcode_data = get_barcode_data(item_code=item.name)
+		if barcode_data and len(barcode_data.get(item.name)) == 1:
+			ret["barcode"] = barcode_data.get(item.name)[0]
+
 		return ret
 
 	@frappe.whitelist()
diff --git a/erpnext/stock/doctype/stock_entry/test_stock_entry.py b/erpnext/stock/doctype/stock_entry/test_stock_entry.py
index 9d7e3ba..34b4ac2 100644
--- a/erpnext/stock/doctype/stock_entry/test_stock_entry.py
+++ b/erpnext/stock/doctype/stock_entry/test_stock_entry.py
@@ -110,6 +110,12 @@
 		self._test_auto_material_request("_Test Item")
 		self._test_auto_material_request("_Test Item", material_request_type="Transfer")
 
+	def test_barcode_item_stock_entry(self):
+		item_code = make_item("_Test Item Stock Entry For Barcode", barcode="BDD-1234567890")
+
+		se = make_stock_entry(item_code=item_code, target="_Test Warehouse - _TC", qty=1, basic_rate=100)
+		self.assertEqual(se.items[0].barcode, "BDD-1234567890")
+
 	def test_auto_material_request_for_variant(self):
 		fields = [{"field_name": "reorder_levels"}]
 		set_item_variant_settings(fields)
diff --git a/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.js b/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.js
index 4a85360..8532b60 100644
--- a/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.js
+++ b/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.js
@@ -81,6 +81,18 @@
 		if (frm.doc.company) {
 			frm.trigger("toggle_display_account_head");
 		}
+
+		frm.events.set_fields_onload_for_line_item(frm);
+	},
+
+	set_fields_onload_for_line_item(frm) {
+		if (frm.is_new() && frm.doc?.items && cint(frappe.user_defaults?.use_serial_batch_fields) === 1) {
+			frm.doc.items.forEach((item) => {
+				if (!item.serial_and_batch_bundle) {
+					frappe.model.set_value(item.doctype, item.name, "use_serial_batch_fields", 1);
+				}
+			});
+		}
 	},
 
 	scan_barcode: function (frm) {
@@ -155,6 +167,9 @@
 
 							item.qty = item.qty || 0;
 							item.valuation_rate = item.valuation_rate || 0;
+							item.use_serial_batch_fields = cint(
+								frappe.user_defaults?.use_serial_batch_fields
+							);
 						});
 						frm.refresh_field("items");
 					},
@@ -298,6 +313,10 @@
 		if (!item.warehouse && frm.doc.set_warehouse) {
 			frappe.model.set_value(cdt, cdn, "warehouse", frm.doc.set_warehouse);
 		}
+
+		if (item.docstatus === 0 && cint(frappe.user_defaults?.use_serial_batch_fields) === 1) {
+			frappe.model.set_value(item.doctype, item.name, "use_serial_batch_fields", 1);
+		}
 	},
 
 	add_serial_batch_bundle(frm, cdt, cdn) {
diff --git a/erpnext/stock/get_item_details.py b/erpnext/stock/get_item_details.py
index ee2f9cb..247ab1f 100644
--- a/erpnext/stock/get_item_details.py
+++ b/erpnext/stock/get_item_details.py
@@ -490,12 +490,21 @@
 		out["barcode"] = barcode_data.get(out.item_code)[0]
 
 
-def get_barcode_data(items_list):
+def get_barcode_data(items_list=None, item_code=None):
 	# get item-wise batch no data
 	# example: {'LED-GRE': [Batch001, Batch002]}
 	# where LED-GRE is item code, SN0001 is serial no and Pune is warehouse
 
 	itemwise_barcode = {}
+	if not items_list and item_code:
+		_dict_item_code = frappe._dict(
+			{
+				"item_code": item_code,
+			}
+		)
+
+		items_list = [frappe._dict(_dict_item_code)]
+
 	for item in items_list:
 		barcodes = frappe.db.get_all("Item Barcode", filters={"parent": item.item_code}, fields="barcode")