diff --git a/erpnext/stock/doctype/item/item.py b/erpnext/stock/doctype/item/item.py
index 8ede955..3abeecd 100644
--- a/erpnext/stock/doctype/item/item.py
+++ b/erpnext/stock/doctype/item/item.py
@@ -107,6 +107,7 @@
 		self.validate_variant_attributes()
 		self.validate_variant_based_on_change()
 		self.validate_fixed_asset()
+		self.clear_retain_sample()
 		self.validate_retain_sample()
 		self.validate_uom_conversion_factor()
 		self.validate_customer_provided_part()
@@ -209,6 +210,13 @@
 			frappe.throw(_("{0} Retain Sample is based on batch, please check Has Batch No to retain sample of item").format(
 				self.item_code))
 
+	def clear_retain_sample(self):
+		if not self.has_batch_no:
+			self.retain_sample = None
+
+		if not self.retain_sample:
+			self.sample_quantity = None
+
 	def add_default_uom_in_conversion_factor_table(self):
 		if not self.is_new() and self.has_value_changed("stock_uom"):
 			self.uoms = []
diff --git a/erpnext/stock/doctype/item/test_item.py b/erpnext/stock/doctype/item/test_item.py
index 669cabc..c12200b 100644
--- a/erpnext/stock/doctype/item/test_item.py
+++ b/erpnext/stock/doctype/item/test_item.py
@@ -656,6 +656,20 @@
 		make_stock_entry(qty=1, item_code=item.name, target="_Test Warehouse - _TC", posting_date = add_days(today(), 5))
 		self.consume_item_code_with_differet_stock_transactions(item_code=item.name)
 
+	@change_settings("Stock Settings", {"sample_retention_warehouse": 0})
+	def test_retain_sample(self):
+		frappe.db.set_single_value('Stock Settings', 'sample_retention_warehouse', '_Test Retain Sample Warehouse')
+		item = make_item("_TestRetainSample", {'has_batch_no': 1, 'retain_sample': 1, 'sample_quantity': 1})
+
+		self.assertEqual(item.has_batch_no, 1)
+		self.assertEqual(item.retain_sample, 1)
+		self.assertEqual(item.sample_quantity, 1)
+
+		item.has_batch_no = None
+		item.save()
+		self.assertEqual(item.retain_sample, None)
+		self.assertEqual(item.sample_quantity, None)
+		item.delete()
 
 	def consume_item_code_with_differet_stock_transactions(self, item_code, warehouse="_Test Warehouse - _TC"):
 		from erpnext.accounts.doctype.sales_invoice.test_sales_invoice import create_sales_invoice
diff --git a/erpnext/stock/doctype/warehouse/test_records.json b/erpnext/stock/doctype/warehouse/test_records.json
index e128558..1b1e905 100644
--- a/erpnext/stock/doctype/warehouse/test_records.json
+++ b/erpnext/stock/doctype/warehouse/test_records.json
@@ -60,5 +60,12 @@
   "warehouse_name": "_Test Warehouse Group-C2",
   "is_group": 0,
   "parent_warehouse": "_Test Warehouse Group - _TC"
+ },
+  {
+  "company": "_Test Company",
+  "doctype": "Warehouse",
+  "warehouse_name": "_Test Retain Sample Warehouse",
+  "parent_warehouse": "_Test Warehouse Group - _TC",
+  "is_group": 0
  }
 ]