diff --git a/erpnext/selling/doctype/quotation/quotation.py b/erpnext/selling/doctype/quotation/quotation.py
index 61c0b8a..548813d 100644
--- a/erpnext/selling/doctype/quotation/quotation.py
+++ b/erpnext/selling/doctype/quotation/quotation.py
@@ -27,6 +27,7 @@
 		self.set_status()
 		self.validate_uom_is_integer("stock_uom", "qty")
 		self.validate_valid_till()
+		self.validate_shopping_cart_items()
 		self.set_customer_name()
 		if self.items:
 			self.with_items = 1
@@ -49,6 +50,26 @@
 		if self.valid_till and getdate(self.valid_till) < getdate(self.transaction_date):
 			frappe.throw(_("Valid till date cannot be before transaction date"))
 
+	def validate_shopping_cart_items(self):
+		if self.order_type != "Shopping Cart":
+			return
+
+		for item in self.items:
+			has_web_item = frappe.db.exists("Website Item", {"item_code": item.item_code})
+
+			# If variant is unpublished but template is published: valid
+			template = frappe.get_cached_value("Item", item.item_code, "variant_of")
+			if template and not has_web_item:
+				has_web_item = frappe.db.exists("Website Item", {"item_code": template})
+
+			if not has_web_item:
+				frappe.throw(
+					_("Row #{0}: Item {1} must have a Website Item for Shopping Cart Quotations").format(
+						item.idx, frappe.bold(item.item_code)
+					),
+					title=_("Unpublished Item"),
+				)
+
 	def has_sales_order(self):
 		return frappe.db.get_value("Sales Order Item", {"prevdoc_docname": self.name, "docstatus": 1})
 
diff --git a/erpnext/selling/doctype/quotation/test_quotation.py b/erpnext/selling/doctype/quotation/test_quotation.py
index b44fa5e..6f0b381 100644
--- a/erpnext/selling/doctype/quotation/test_quotation.py
+++ b/erpnext/selling/doctype/quotation/test_quotation.py
@@ -130,6 +130,15 @@
 		quotation.submit()
 		self.assertRaises(frappe.ValidationError, make_sales_order, quotation.name)
 
+	def test_shopping_cart_without_website_item(self):
+		if frappe.db.exists("Website Item", {"item_code": "_Test Item Home Desktop 100"}):
+			frappe.get_last_doc("Website Item", {"item_code": "_Test Item Home Desktop 100"}).delete()
+
+		quotation = frappe.copy_doc(test_records[0])
+		quotation.order_type = "Shopping Cart"
+		quotation.valid_till = getdate()
+		self.assertRaises(frappe.ValidationError, quotation.validate)
+
 	def test_create_quotation_with_margin(self):
 		from erpnext.selling.doctype.quotation.quotation import make_sales_order
 		from erpnext.selling.doctype.sales_order.sales_order import (